Abstracts: Annual Scientific Meeting of the European College of Veterinary Ophthalmologists, Helsinki, Finland May 28–31, 2015

ORAL

Use of porcine urinary bladder acellular matrix for corneal repair in dogs and cats

O. Balland (Centre Hospitalier Vétérinaire, Ludres, France)
A.S. Poinsard (Centre Hospitalier Vétérinaire, Ludres, France)
F. Famose (Clinique vétérinaire des Acacias, Blagnac, France)
F. Goulle (Clinique Vétérinaire, Eysines, France)
P.F. Isard (Centre Hospitalier Vétérinaire, Saint-Martin Bellevue, France)
I. Mathieson (Eyevet Referrals, Sutton Weaver, Cheshire, UK)
T. Dulaurent (Centre Hospitalier Vétérinaire, Saint-Martin Bellevue, France)

Purpose: To describe the use of a porcine bladder acellular matrix in the surgical manage- ment of deep corneal ulcers in dogs and cats and feline corneal sequestra. Methods: Twenty-five dogs and three cats with corneal ulcers affecting more than half of the corneal thickness as observed by slit-lamp biomicroscopy, two dogs with corneal perforations and seven cats with sequestra were included. The necrotic material was removed by keratectomy. The collagen graft (Acellvet ») was sutured into the keratectomy bed using a continuous suture pattern of Polyglactine (9-0). A nictitating membrane flap (NMF) was placed to pro- tect the cornea and to prevent graft dehydration. Post-operative treatments consisted of topi- cal tobramycin, atropine and systemic amoxicillin-clavulanic acid. The NMF was removed on day 7 for the 9 most severely affected corneas for complete evaluation. The NMF was then replaced. On day 18 the NMF was removed in all cases. All patients were examined on days 7, 18, 45, 90. Epithelialization of the biomaterial was monitored using fluorescein. Re- sults: In two dogs, a conjunctival graft was performed at day 7 to cover the biomaterial graft, because of progression of a collagenolytic process. Epithelialization was complete at day 18 in 34/37 cases. Corneal opacity was dense in eight eyes, moderate in 21, and absent in 6 at day 90. Vision tests were positive in 35 eyes. Conclusion: The results of our study are comparable to those of other studies describing the use of other collagen xenografts in the surgical management of corneal defects of various origins.